کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10968785 | 1102905 | 2011 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Live vaccine of viral hemorrhagic septicemia virus (VHSV) for Japanese flounder at fish rearing temperature of 21 °C instead of Poly(I:C) administration
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The process of “Poly(I:C) immunization” involves immunization of fish with a pathogenic live virus, followed by administration of Poly(I:C), which induces a transient, non-specific antiviral state. As a result, fish in an antiviral state survive the initial immunization with live virus. Moreover, these fish are able to mount a specific protective immune response against the injected pathogenic virus. In the present study, we investigated the optimum temperature for Poly(I:C) immunization of Japanese flounder Paralichthys olivaceus with live viral hemorrhagic septicemia virus (VHSV). It was revealed that the optimum temperature was around at 17 °C for Poly(I:C) immunization in Japanese flounder. Furthermore, the protection efficacy of Poly(I:C) immunization was significantly decreased by elevation of fish rearing temperature, and no efficacy was observed at a fish rearing temperature of 25 °C. Interestingly, no mortality by VHSV infection was observed in fish reared at 21 °C and 25 °C even when those fish were not administered Poly(I:C). All of the survivors from the first VHSV-challenge at 21 °C were strongly protected from re-challenge with VHSV. However, almost all of the survivors (â¥82.6%) from the first challenge at 25 °C were lost by the second challenge with VHSV. It was thus concluded that by rearing fish at 21 °C and challenging with live VHSV, it is possible to induce strong specific immunity in Japanese flounder without Poly(I:C) administration.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 29, Issue 46, 26 October 2011, Pages 8397-8404
Journal: Vaccine - Volume 29, Issue 46, 26 October 2011, Pages 8397-8404
نویسندگان
Toyohiko Nishizawa, Ikuo Takami, Minji Yang, Myung-Joo Oh,