UV-inactivated vaccinia virus (VV) in a multi-envelope DNA-VV-protein (DVP) HIV-1 vaccine protects macaques from lethal challenge with heterologous SHIV

► UV-inactivated vaccinia virus is a successful HIV-1 vaccine vehicle. ► A multi-envelope HIV-1 vaccine protects against heterologous SHIV in macaques. ► DNA-vaccinia virus-protein prime-boost vaccine protects against heterologous SHIV.