Protective immune responses to biolistic DNA vaccination of Brugia malayi abundant larval transcript-2

► Protective responses to a DNA vaccine were compared after 2 delivery methods. ► Brugia malayi abundant larval transcript-2 DNA was used as the vaccine candidate. ► Gene gun delivery of BmALT-2 DNA vaccine was optimized in this study. ► Findings show that gene gun delivery of BmALT-2 DNA elicits significant protection. ► Gene gun approach required only 20 fold less DNA compared to intradermal approach.