Lymphocyte-polarized dendritic cells are highly effective in inducing tumor-specific CTLs

► Lymphocytes, or their supernatants induce maturation and type-1 polarization of DCs. ► Lymphocyte-matured DC1s produce IL-12p70 and CXCL10 upon subsequent CD40 ligation. ► Lymphocyte-matured DC1s showed enhanced CCR7 expression and migration to CCL21. ► Lymphocyte-matured DC1s induce CTLs against tumor-associated antigens. ► TNFα/IFNγ-secreting T cells limit the need for DC1-polarizing cytokines.