کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10969556 1102967 2010 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Evidence of Chlamydophila abortus vaccine strain 1B as a possible cause of ovine enzootic abortion
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Evidence of Chlamydophila abortus vaccine strain 1B as a possible cause of ovine enzootic abortion
چکیده انگلیسی
Chlamydophila abortus, the agent of ovine enzootic abortion (OEA), is a major cause of lamb mortality worldwide. Disease can be controlled through the use of vaccines based on the 1B temperature-sensitive mutant strain of C. abortus. This study investigated suspected OEA cases across Scotland for the presence of the 1B strain by analysis of recently identified unique point mutations (9). Thirty-five cases were C. abortus-positive and 14 came from vaccinated flocks. Analysis of single nucleotide polymorphisms by PCR-RFLP and sequence analysis revealed the presence of point mutations consistent with the presence of the 1B vaccine strain in 5 of these 14 samples. Quantitative real-time PCR revealed comparable numbers of genome copies of the 1B strain in infected placentas to those present following wild-type infection. This study is the first to demonstrate the presence of the 1B vaccine strain in the placentas of OEA cases and suggests a probable causal role in the disease.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 28, Issue 35, 9 August 2010, Pages 5657-5663
نویسندگان
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