کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10969681 1102977 2010 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
An in vitro assay system as a potential replacement for the histamine sensitisation test for acellular pertussis based combination vaccines
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
An in vitro assay system as a potential replacement for the histamine sensitisation test for acellular pertussis based combination vaccines
چکیده انگلیسی
The histamine sensitisation test (HIST) for pertussis toxin is currently an official batch release test for acellular pertussis containing combination vaccines in Europe and North America. However, HIST, being a lethal endpoint assay, often leads to repeated tests due to large variations in test performance. Although a more precise HIST test based on measurement of temperature reduction after the histamine challenge is used in Asian countries, this test still uses animals. An in vitro test system based on a combination of enzyme coupled-HPLC and carbohydrate-binding assays with results analysed by a mathematical formula showed a good agreement with the in vivo HIST results based on measurement of temperature reduction after histamine challenge. The new in vitro test system was shown to be a potential alternative to the current in vivo HIST.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 28, Issue 21, 7 May 2010, Pages 3714-3721
نویسندگان
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