کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10969701 | 1102981 | 2009 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Mannosamine-biotin as a novel masking agent for coating IgG for immune response silencing and augmentation of antibody-antigen interaction
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کلمات کلیدی
MBATNBSAHIgGTrinitrophenylECLTNPHRPHuman IgG - IgG انسانیPassive immunization - ایمنی غیر فعالenhanced chemiluminescence - بهبود شیمیایی لومنtetanus toxoid - توکسوئین تانتانسCoating agent - عامل پوششAntibody - پادتَن یا آنتیبادیImmune response - پاسخ یا واکنش ایمنیHorseradish peroxidase - پراکسیداز هوررادیشpolyethylene glycol - پلی اتیلن گلیکولPEG - پلیاتیلن گلیکول carboxyfluorescein succinimidyl ester - کربوکسیفلوورسسین سوکسینیمیدیل استر
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A variety of protein-coating procedures are used to modify proteins' properties. The principle coating agent used is PEGylation, in which proteins are coated by conjunction to polyethylene glycol (PEG). In the present study, we describe a novel approach that makes use of small molecules with multifunctional groups as the protein-coating agent. The new coating molecule was produced by reacting two endogenous molecules, mannosamine and biotin, to form mannose-biotin adducts (MBA). hIgG was coated with MBA at various MBA/protein ratios. The immunogenicity of MBA-coated hIgG was tested in chickens. A dose-responsive effect of MBA/hIgG ratio on immune response suppression was detected, with an optimal masking effect at a 12:1 ratio. The immune response to MBA-coated hIgG was about eightfold lower than that to PEG-coated hIgG. MBA also increased antibody-antigen-binding affinity, and decreased recognition of the Fc domain of MBA-coated hIgG by Fc receptor and secondary antibodies. While the PEG molecule consists of inert repeating units of ethylene oxide with no additional functional group to allow for potentially desirable modifications, the MBA has several functional groups, including vicinal hydroxyls, which can easily be converted to active residues such as aldehydes or carboxyls. This may be of importance for developing passive immunizations or for achieving tolerance of the immune response to an immunogenic molecule or virus. In summary, we developed a new protein-coating molecule with the ability to mask foreign antigens and in the case of antibodies, to enhance activity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 27, Issue 49, 16 November 2009, Pages 6869-6876
Journal: Vaccine - Volume 27, Issue 49, 16 November 2009, Pages 6869-6876
نویسندگان
Jacob Vaya, Elina Aizenshtein, Soliman Khatib, Tal Gefen, Michael Fassler, Ramadan Musa, Simi Krispel, Jacob Pitcovski,