کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10969751 | 1102986 | 2009 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Recombinant outer membrane vesicles to augment antigen-specific live vaccine responses
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Recombinant outer membrane vesicles to augment antigen-specific live vaccine responses Recombinant outer membrane vesicles to augment antigen-specific live vaccine responses](/preview/png/10969751.png)
چکیده انگلیسی
Release of outer membrane vesicles is a common feature of Gram negative bacteria. There is growing interest in the use of these vesicles in the development of affordable vaccines. However, to exploit their full potential a convenient system to generate recombinant vesicles would be highly desirable. Here, we report the design of a versatile system for preparation of recombinant outer membrane vesicles based on an engineered autotransporter. Two model vaccine antigens of Leishmania were expressed as fusion proteins with the transporter domain of AIDA, the Escherichia coli adhesin involved in diffuse adhesion. Single subcutaneous injections of recombinant vesicles boosted vaccine antigen-specific antibody responses in mice primed with live recombinant salmonella vaccines by 6-40-fold. The results further show an expansion of only the vaccine antigen-specific antibody response indicating a great potential of this approach for prime boost vaccination strategies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 27, Issue 48, 12 November 2009, Pages 6748-6754
Journal: Vaccine - Volume 27, Issue 48, 12 November 2009, Pages 6748-6754
نویسندگان
Juliane Schroeder, Toni Aebischer,