Development of a baited oral vaccine for use in reservoir-targeted strategies against Lyme disease

► We have developed a reservoir-targeted baited vaccine against Lyme disease. ► The vaccine is stable in oral baits under various environment-simulated conditions. ► A single dose of vaccine protects Peromyscus mice against infected tick bites. ► A single dose vaccine protects ticks from infection during feeding on carrier mice. ► Live, in vivo imaging was used to optimize bait composition.