کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10970128 | 1103025 | 2009 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A novel technology for the production of a heterologous lipoprotein immunogen in high yield has implications for the field of vaccine design
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موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
We have developed a novel platform technology that can express high levels of recombinant lipoproteins with intrinsic adjuvant properties. In this study, Ag473 (a lipoprotein from Neisseria meningitidis) can be produced in high yields using Escherichia coli strain C43 (DE3). After testing a non-lipoimmunogen (E3, from dengue virus) fused with different lipid signal peptides from other lipoproteins as well as Ag473 fragments of different lengths, we identified that the fusion sequence has to contain at least the N-terminal 40 residues, D1, of Ag473 to achieve high expression levels of the recombinant lipo-immunogen (rlipo-D1E3). The rlipo-D1E3 was found to elicit stronger anti-E3 and virus neutralizing antibody responses in animal studies than those from rE3 alone or rE3 formulated with alum adjuvant. These results have successfully demonstrated the merit of lipo-immunogens for novel vaccine development.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 27, Issue 9, 25 February 2009, Pages 1400-1409
Journal: Vaccine - Volume 27, Issue 9, 25 February 2009, Pages 1400-1409
نویسندگان
Hsin-Wei Chen, Shih-Jen Liu, Hsueh-Hung Liu, Yan Kwok, Chang-Ling Lin, Li-Hsiu Lin, Mei-Yu Chen, Jy-Ping Tsai, Li-Sheng Chang, Fang-Feng Chiu, Li-wei Lai, Wei-Cheng Lian, Chiou-Ying Yang, Shih-Yang Hsieh, Pele Chong, Chih-Hsiang Leng,