کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10970128 1103025 2009 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A novel technology for the production of a heterologous lipoprotein immunogen in high yield has implications for the field of vaccine design
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
A novel technology for the production of a heterologous lipoprotein immunogen in high yield has implications for the field of vaccine design
چکیده انگلیسی
We have developed a novel platform technology that can express high levels of recombinant lipoproteins with intrinsic adjuvant properties. In this study, Ag473 (a lipoprotein from Neisseria meningitidis) can be produced in high yields using Escherichia coli strain C43 (DE3). After testing a non-lipoimmunogen (E3, from dengue virus) fused with different lipid signal peptides from other lipoproteins as well as Ag473 fragments of different lengths, we identified that the fusion sequence has to contain at least the N-terminal 40 residues, D1, of Ag473 to achieve high expression levels of the recombinant lipo-immunogen (rlipo-D1E3). The rlipo-D1E3 was found to elicit stronger anti-E3 and virus neutralizing antibody responses in animal studies than those from rE3 alone or rE3 formulated with alum adjuvant. These results have successfully demonstrated the merit of lipo-immunogens for novel vaccine development.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 27, Issue 9, 25 February 2009, Pages 1400-1409
نویسندگان
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