کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10970437 | 1103134 | 2008 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Universal antibodies and their applications to the quantitative determination of virtually all subtypes of the influenza A viral hemagglutinins
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Universal antibodies and their applications to the quantitative determination of virtually all subtypes of the influenza A viral hemagglutinins Universal antibodies and their applications to the quantitative determination of virtually all subtypes of the influenza A viral hemagglutinins](/preview/png/10970437.png)
چکیده انگلیسی
The fusion peptide is the only universally conserved sequence in the hemagglutinins of all 16 subtypes of influenza A and two genetic lineages of influenza B viruses. Here, peptides selected by bioinformatics approach were modified and conjugated to overcome serious technical hurdles such as the high hydrophobicity and weak immunogenicity of the viral fusion peptides. Antibodies generated against fusion peptides demonstrated remarkable specificity against the viral sequences and robustness of quantitatively analyzing the viral hemagglutinins even under stringent conditions. As quantitatively revealed by antibody-binding experiments, the fusion peptides of diverse hemagglutinins are exposed to the same degree upon unfolding at neutral pH to the physiologically fusogenic state. To our knowledge, this is the first report on the quantitative determination of virtually all influenza vaccines using a single universal antibody.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 26, Issue 48, 11 November 2008, Pages 6068-6076
Journal: Vaccine - Volume 26, Issue 48, 11 November 2008, Pages 6068-6076
نویسندگان
Stella Chun, Changgui Li, Gary Van Domselaar, Junzhi Wang, Aaron Farnsworth, Xiaoyu Cui, Harold Rode, Terry D. Cyr, Runtao He, Xuguang Li,