کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10970469 1103170 2007 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Protective immunity conferred by recombinant Pasteurella multocida lipoprotein E (PlpE)
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Protective immunity conferred by recombinant Pasteurella multocida lipoprotein E (PlpE)
چکیده انگلیسی
The genes encoding Pasteurella multocida lipoprotein E (PlpE) and lipoprotein B (PlpB) were cloned from P. multocida strain X-73 (serotype A:1) and expressed in Escherichia coli. The protective immunity conferred by recombinant PlpE (r-PlpE) and PlpB (r-PlpB) on mice and chickens was evaluated. The results showed that mice immunized with 10 μg of purified r-PlpE were protected (80-100% survival rate) against challenge infection with 10 or 20 LD50 of P. multocida strains X-73 (serotype A:1), P-1059 (serotype A:3) and P-1662 (serotype A:4). In contrast, mice immunized with r-PlpB were not protected. Chickens immunized with 100 μg of purified r-PlpE were protected (63-100% survival rate) against lethal challenge infection with strains X-73 and P-1662, whereas those immunized with r-PlpB were not. Sequence analyses showed that PlpE from different strains of P. multocida exhibited 90.8-100% sequence identity to each other, suggesting that PlpE might serve as a cross-protective antigen. This is the first report of a recombinant P. multocida antigen that confers cross protection on animals.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 25, Issue 21, 22 May 2007, Pages 4140-4148
نویسندگان
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