Measuring the blockade of malaria transmission - An analysis of the Standard Membrane Feeding Assay

► Malaria transmission blocking candidates need to be prioritized to transfer the best to the field. ► SMFA analyses vary between laboratories and efficacy estimates are often underpowered and highly uncertain. ► The effectiveness of a TBI candidate to reduce mosquito infectivity depends on parasite exposure. ► We show that the relationship between oocyst prevalence and intensity is predictable. ► This will allow laboratory results to be extrapolated to different field situations.