کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
11001783 | 1051446 | 2018 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
2.8-Ã
crystal structure of Escherichia coli YidC revealing all core regions, including flexible C2 loop
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
YidC/Alb3/Oxa1 family proteins are involved in the insertion and assembly of membrane proteins. The core five transmembrane regions of YidC, which are conserved in the protein family, form a positively charged cavity open to the cytoplasmic side. The cavity plays an important role in membrane protein insertion. In all reported structural studies of YidC, the second cytoplasmic loop (C2 loop) was disordered, limiting the understanding of its role. Here, we determined the crystal structure of YidC including the C2 loop at 2.8â¯Ã
resolution with R/Rfreeâ¯=â¯21.8/27.5. This structure and subsequent molecular dynamics simulation indicated that the intrinsic flexible C2 loop covered the positively charged cavity. This crystal structure provides the coordinates of the complete core region including the C2 loop, which is valuable for further analyses of YidC.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 505, Issue 1, 20 October 2018, Pages 141-145
Journal: Biochemical and Biophysical Research Communications - Volume 505, Issue 1, 20 October 2018, Pages 141-145
نویسندگان
Yoshiki Tanaka, Akiya Izumioka, Aisyah Abdul Hamid, Akira Fujii, Takamitsu Haruyama, Arata Furukawa, Tomoya Tsukazaki,