کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
11016668 1773884 2018 35 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Impact of hydrodynamics on iPSC-derived cardiomyocyte differentiation processes
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Impact of hydrodynamics on iPSC-derived cardiomyocyte differentiation processes
چکیده انگلیسی
Laser Doppler Anemometry and Fast Fourier Transform analysis were used to identify characteristic flow frequencies under different agitation modes. Intermittent agitation resulted in a pattern of low intensity frequencies at reactor scale that could be controlled by varying three identified time components: rotational speed, interval and dwell times. A proof of concept biological study was undertaken, tuning the hydrodynamic environment through variation of dwell time based on the engineering study findings and a significant improvement in CM yield was obtained. This work introduces the concept of fine-tuning the physical hydrodynamic cues within a three-dimensional flow system to improve cardiomyocyte differentiation of iPSC.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 287, 10 December 2018, Pages 18-27
نویسندگان
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