کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
11024854 1701066 2019 27 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
FTIR-ATR determination of protein content to evaluate whey protein concentrate adulteration
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
FTIR-ATR determination of protein content to evaluate whey protein concentrate adulteration
چکیده انگلیسی
We evaluated the potential application of Fourier-Transformed Infrared spectroscopy using Attenuated Total Reflectance (FTIR-ATR) to characterize and detect adulteration of whey protein concentrate (WPC). Samples were adulterated by substituting WPC by milk whey powder (MWP). Principal Component Analysis (PCA) was used to characterize the spectra. Partial Least Squares (PLS) regressions were applied to model and predict the protein content and the amount in grams of WPC and MWP in the samples. In WPC, Amide I and II bands showed decreasing trend with the protein content of the samples. The carbohydrate region between 1160 and 1000 cm−1 showed spectra with opposite behavior of the Amide bands, evincing the ν CO peak (1030 cm−1) that increases as MWP was added. In PCA, PC1 (64.5%) and PC2 (18.8%) described above 80% of total variance of the spectra. PC1 variance was most influenced by Amide I, II and carbohydrate regions. In all cases, PLS showed low prediction errors, high precision and coefficients of determination of the global fit above 0.99. Therefore, the association of FTIR-ATR spectroscopy with multivariate approaches revealed strong potential to detect adulteration in nutritional supplements and high accuracy to predict simultaneously the protein content and mass of WPC and MWP added.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: LWT - Volume 99, January 2019, Pages 166-172
نویسندگان
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