کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
11025929 | 1666471 | 2018 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Optimal human iPS cell culture method for efficient hepatic differentiation
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کلمات کلیدی
FBSHLCsOSMMEFHGFALBGAPDHHCMFGFAlbumin - آلبومینdefinitive endoderm - اندودرم قطعیoncostatin M - اوکتواستاتین مDifferentiation - تفکیکfetal bovine serum - سرم جنین گاوES cells - سلول ESiPS cells - سلول های IPSHuman induced pluripotent stem cells - سلول های بنیادی تکامل یافته القا شده توسط انسانInduced pluripotent stem cells - سلول های بنیادی پرتوان القاییhepatocyte-like cells - سلول های مشابه سلول های هپاتوستیکEmbryonic stem cells - سلولهای بنیادی جنینیHepatocyte growth factor - عامل رشد هپاتوسیتfibroblast growth factor - فاکتور رشد فیبروبلاستBMP - مدیریت فرایند کسب و کارmouse embryonic fibroblast - موش فیبروبلاست جنینیHepatocyte - هپاتوسیتBone morphogenetic protein - پروتئین مورفوژنیک استخوانglyceraldehyde 3-phosphate dehydrogenase - گلیسرولیدید 3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Hepatocyte-like cells differentiated from human iPS cells are expected to be utilized in pharmaceutical research and regenerative medicine. Recently, various culture methods for human iPS cell maintenance have been developed. However, it is not well known whether human iPS cell maintenance method affects hepatic differentiation potency. In this study, we cultured human iPS cells using four maintenance methods: ReproStem medium with feeder cells (mouse embryonic fibroblasts), AK02N medium with iMatrix-511 (E8 fragments of laminin511), Essential 8 medium with Vitronectin N (N-terminal domain of vitronectin), TeSR-E8 medium with Vitronectin XF (xeno-free vitronectin). Then, these human iPS cells were differentiated into the hepatocyte-like cells. Interestingly, the gene expression levels of definitive endoderm markers in the definitive endoderm cells generated from human iPS cells cultured with ReproStem or TeSR-E8 medium were higher than those in other groups. The gene expression level of foregut marker, HHEX, in the definitive endoderm cells generated from human iPS cells cultured with ReproStem medium was higher than that in other groups. Consistently, the expression levels of hepatocyte markers, albumin and urea secretion capacity, and CYP3A4 activity in the hepatocyte-like cells generated from human iPS cells cultured with ReproStem medium were higher than those in the other groups. Our data indicated that the most suitable human iPS cell maintenance method for efficient hepatic differentiation was the on-feeder method which uses mouse embryonic fibroblasts, but not feeder-free methods. In conclusion, human iPS cell maintenance method largely affects hepatic differentiation potency.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Differentiation - Volume 104, NovemberâDecember 2018, Pages 13-21
Journal: Differentiation - Volume 104, NovemberâDecember 2018, Pages 13-21
نویسندگان
Nobumasa Matoba, Tomoki Yamashita, Kazuo Takayama, Fuminori Sakurai, Hiroyuki Mizuguchi,