کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
11029029 | 1646658 | 2018 | 28 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Biofilms of Candida albicans and Streptococcus sanguinis and their susceptibility to antimicrobial effects of photodynamic inactivation
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کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
پزشکی و دندانپزشکی (عمومی)
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چکیده انگلیسی
This study evaluated the effects of photodynamic inactivation (PDI) on single and multi-species biofilms, compounds by Candida albicans and Streptococcus sanguinis. Biofilms were formed, on microplate of 96 wells, by suspensions of C. albicans (ATCC 18804) and S. sanguinis (ATCC 7073) adjusted in 107 cells/mL, followed by incubation of 48â¯h (with 5% CO2). The effects of the photosensitizer erythrosine (ER) at 400 μM for 5â¯min and green light-emitting diode (LED - 532â¯Â±â¯10â¯nm) for 3â¯min, alone and conjugated, were evaluated. After normality test, results was analysed by Tukey´s test (Pâ¯<â¯0.05). PDI group promoted reductions of 1.07 and 0.39 log10, respectively, in biofilms of C. albicans alone and in association with S. sanguinis. Biofilms of S. sanguinis alone were more sensitive, with reduction of 4.48 log10. When in association with the yeast, S. sanguinis have a decrease of 2.67 log10. SEM analysis revealed a decrease in bacterial and fungal structures of biofilms treated with PDI. In conclusion PDI promoted significant microbial reductions in both species of microorganisms grown on mixed biofilms. This study is one of the pioneers to evaluate the antimicrobial action of PDI on biofilms of S. sanguinis and C. albicans, demonstrating a way to control these microorganisms of clinical importance.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Photodiagnosis and Photodynamic Therapy - Volume 24, December 2018, Pages 95-101
Journal: Photodiagnosis and Photodynamic Therapy - Volume 24, December 2018, Pages 95-101
نویسندگان
Ana Luiza do Rosário Palma, Lucas de Paula-Ramos, Nádia Domingues, Graziella Nuernberg Back-Brito, Luciane Dias de Oliveira, Cristiane Aparecida Pereira, Antonio Olavo Cardoso Jorge,