کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
11033763 | 1570071 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Genomic transcriptional response to 20-hydroxyecdysone in the fat body of silkworm, Bombyx mori
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Insect growth and development are primarily controlled by two major hormones, juvenile hormone and molting hormone. 20-Hydroxyecdysone is the most active form of the molting hormone. Although intensive studies have been performed on its biological function and action mechanism, it is still unknown how many genes are directly or indirectly regulated by the molting hormone. Here, we analyzed the genomic transcriptional response to 20-hydroxyecdysone in the fat body of silkworm, by using high-throughput Illumina sequencing technology and bioinformatics tools. In total, 606 differentially expressed genes with 347 up-regulated and 259 down-regulated were detected. The 606 differentially expressed genes were significantly enriched in 118 GO terms, i.e. biological process (68), molecular function (37) and cellular component (13). The KEGG analysis revealed that the significantly enriched pathways were mainly focused on the metabolic processes. The differentially expressed genes were further aligned to the functionally verified sequences of B. mori in the NCBI database, and a total of 43 functional sequences were identified, of which 23 genes were down-regulated and 20 genes were up-regulated. The up-regulated genes mainly relate to metamorphosis, immune response and protein synthesis. RT-qPCR analysis further validated the correctness of the digital gene expression data. Our study gives an overall view of the regulating effect of 20-hydroxyecdysone on the whole-genome transcript expression in the silkworm, provides useful dataset, and will be helpful for the further studies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene Reports - Volume 13, December 2018, Pages 134-140
Journal: Gene Reports - Volume 13, December 2018, Pages 134-140
نویسندگان
ShuoHao Huang, HuanHuan Yang, XingXing Chen, JianYun Zhang, LongQuan Huang,