کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1162681 | 1490895 | 2016 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: A two-photon fluorescent turn-on probe for imaging of SO2 derivatives in living cells and tissues A two-photon fluorescent turn-on probe for imaging of SO2 derivatives in living cells and tissues](/preview/png/1162681.png)
• A FRET-based two-photon fluorescent probe for detecting SO2 derivatives was proposed.
• The probe shows high sensitivity, excellent selectivity, rapid response as well as low cytotoxicity.
• The probe was capable of detecting SO2 derivatives up to 170 μm depth in tissues.
SO2 and its derivatives (bisulfite/sulfite) play crucial roles in several physiological processes. Therefore, development of reliable analytical methods for monitoring SO2 and its derivatives in biological systems is very significant. In this paper, a FRET-based two-photon fluorescent turn-on probe, A-HCy, was proposed for specific detection of SO2 derivatives through the bisulfite/sulfite-promoted Michael addition reaction. In this FRET system, an acedan (2-acetyl-6-dialkylaminonaphthalene) moiety was selected as a two-photon donor and a hemicyanine derivative served as both the quencher and the recognition unit for bisulfite/sulfite. A-HCy exhibited excellent selectivity and rapid response to HSO3− with a detection limit of 0.24 μM. More importantly, probe A-HCy was first successfully applied in two-photon fluorescence imaging of biological SO2 derivatives in living cells and tissues, suggesting its great potential for practical application in biological systems.
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Journal: Analytica Chimica Acta - Volume 937, 21 September 2016, Pages 136–142