کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1162775 1490907 2016 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Rapid “one-pot” preparation of polymeric monolith via photo-initiated thiol-acrylate polymerization for capillary liquid chromatography
ترجمه فارسی عنوان
سریع یک پاپا تهیه پلیمر یکپارچه از طریق پلیمراز سازی تیل آریلات آغاز شده برای عکسبرداری برای کروماتوگرافی مایع مویرگی
کلمات کلیدی
پلیمر تک، عکس پلی اورتان تیل آکریلیک آغاز شده، کروماتوگرافی مایع مغناطیسی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• A highly crosslinked polymeric monolith was fast prepared with a multi-acrylate monomer as crosslinker within 5 min.
• The addition of thiol eliminated the major obstacle of oxygen inhibition in the thiol-ene (acrylate/methacrylate) polymerization.
• Poly(ODT-co-DPEPA) monolith exhibited efficient chromatographic performance for separation of alkylbenzenes and tryptic digest of proteins.

A facile approach was exploited for fast preparation of polymer-based monoliths in UV-transparent fused-silica capillaries via “one-pot” photo-initiated thiol-acrylate polymerization reaction of dipentaerythritolpenta-/hexaacrylate (DPEPA) and 1-octadecanethiol (ODT) in the presence of porogenic solvents (1-butanol and ethylene glycol). Due to relative insensitivity of oxygen inhibition in thiol-ene free-radical polymerization, the polymerization could be performed within 5 min. The effects of composition of prepolymerization solution on the morphology and permeability of poly(ODT-co-DPEPA) monoliths were investigated in detail by adjusting the content of monomer and binary porogen ratio. The physical properties of poly(ODT-co-DPEPA) monoliths were characterized by Fourier transform infrared spectroscopy (FT-IR), mercury intrusion porosimetry (MIP) and nitrogen adsorption/desorption measurement. The evaluation of chromatographic performance was carried out by capillary liquid chromatography (cLC). The results indicated that the poly(ODT-co-DPEPA) monolith was homogeneous and permeable, and also possessed a typical reversed-phase retention mechanism in cLC with high efficiency (∼75,000 N m−1) for separation of alkylbenzenes. Eventually, the further separation of tryptic digest of proteins by cLC tandem mass spectrometry (cLC-MS/MS) demonstrated its potential in the analysis of biological samples.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 925, 21 June 2016, Pages 88–96
نویسندگان
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