کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1163078 1490922 2016 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Capillary hydrodynamic chromatography reveals temporal profiles of cell aggregates
ترجمه فارسی عنوان
کروماتوگرافی هیدرودینامیکی کاپیلاریک نشان می دهد که پروفیل های زمانی از ترکیبات سلولی
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• Gravitational sedimentation is coupled on-line with hydrodynamic chromatography.
• Area imaging detection system enables multiplexing hydrodynamic separations.
• On-line and off-line microscopy facilitate peak assignment.
• The approach unravels dynamics of cell aggregates in complex cell suspensions.
• Temporal profiles of microbiomes in probiotic drinks are recorded.

Microbial cells are known to form aggregates. Such aggregates can be found in various matrices; for example, functional drinks. Capillary hydrodynamic chromatography (HDC) enables separation of particles by size using nanoliter-scale volumes of samples. Here we propose an approach based on HDC for characterisation of real samples containing aggregated and non-aggregated bacterial and fungal cells. Separation of cells and cell aggregates in HDC arises from the parabolic flow profile under laminar flow conditions. In the presented protocol, hydrodynamic separation is coupled with different on-line and off-line detectors (light absorption/scattering and microscopy). The method has successfully been applied in the monitoring of dynamic changes in the microbiome of probiotic drinks. Chromatographic profiles of yogurt and kefir samples obtained at different times during fermentation are in a good agreement with microscopic images. Moreover, thanks to the implementation of an area imaging detector, capillary HDC could be multiplexed and used to profile spatial gradients in cell suspensions, which arise in the course of sedimentation of cells and cell aggregates. This result shows compatibility of sedimentation analysis and capillary HDC. We believe that the approach may find applications in the profiling of functional foods and other matrices containing aggregated bioparticles.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 910, 3 March 2016, Pages 75–83
نویسندگان
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