کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1163213 1490928 2016 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Nanogold-penetrated poly(amidoamine) dendrimer for enzyme-free electrochemical immunoassay of cardiac biomarker using cathodic stripping voltammetric method
ترجمه فارسی عنوان
دندریمر پلی (آمیدآمین) نفوذ نانوذرات نفوذی برای ایمونوسیت الکتروشیمیایی آنزیم بیومارکرهای قلبی با استفاده از روش ولتامتریک سات سدیم
کلمات کلیدی
آزمایش ایمونوژن بدون آنزیم، ایمونوسانسور الکتروشیمیایی، نانوذرات طلا، پلی (آمیدآمین) دندریمر، ولتاژ سنجی سلب کاتدیک، میوگلوبین
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• We designed a new voltammetric immunoassay for detection of cardiac biomarker.
• The signal was amplified by nanogold-penetrated poly(amidoamine) dendrimers.
• The encapsulated nanogold particles were used as the signal-generation tag.

Methods based on immunoassays have been developed for cardiac biomarkers, but most involve the low sensitivity and are unsuitable for early disease diagnosis. Herein we design an electrochemical immunoassay for sensitive detection of myoglobin (a cardiac biomarker for acute myocardial infarction) by using nanogold-penetrated poly(amidoamine) dendrimer (AuNP-PAMAM) for signal amplification without the need of natural enzymes. The assay was carried out on the monoclonal mouse anti-myoglobin (capture) antibody-anchored glassy carbon electrode using polyclonal rabbit anti-myoglobin (detection) antibody-labeled AuNP-PAMAM as the signal tag. In the presence of target myoglobin, the sandwiched immunocomplex could be formed between capture antibody and detection antibody. Accompanying AuNP-PAMAM, the carried gold nanoparticles could be directly determined via stripping voltammetric method under acidic conditions. Under optimal conditions, the detectable electrochemical signal increased with the increasing target myoglobin in the sample within a dynamic working range from 0.01 to 500 ng mL−1 with a detection limit of 3.8 pg mL−1. The electrochemical immunoassay also exhibited high specificity and good precision toward target myoglobin. Importantly, our strategy could be applied for quantitative monitoring of myoglobin in human serum specimens, giving well matched results with those obtained from commercialized enzyme-linked immunosorbent assay (ELISA) method.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 904, 21 January 2016, Pages 51–57
نویسندگان
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