کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1163573 1490945 2015 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Enhanced immunoassay for porcine circovirus type 2 antibody using enzyme-loaded and quantum dots-embedded shell–core silica nanospheres based on enzyme-linked immunosorbent assay
ترجمه فارسی عنوان
آزمایش ایمنی پیشرفته برای آنتی بادی تیپ 2 تیروئید کدو با استفاده از آنزیم لود شده و نقاط کوانتومی- انباشته شده سیلیکا سلولهای شل شده بر اساس آنزیم مرتبط با ایمونوسیورسنت
کلمات کلیدی
آزمایش آنزیم ایمونوسیورسانس، ایمونوسنسور، آنزیم، نقاط کوانتومی، نانوسفر سیلیکا
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• A versatile ELISA-based immunoassay for PCV2 antibody was developed.
• Enzyme and CdSe QDs modified SiO2 particles were used to improve sensitivity.
• The simultaneous three ELISA-based techniques enhanced the detection reliability.
• The biosensors strategy could provide a new avenue to ELISA-based sensors.

Boosting the detection sensitivity of enzyme-linked immunosorbent assay (ELISA) is significant to the early clinical diagnosis of various diseases. Here, we developed a versatile immunosensor using silica nanospheres as carriers for sensitive detection of porcine circovirus type 2 (PCV2) antibody. With HRP enzyme covalently immobilized on the silica nanospheres and CdSe nanocrystals embedded inside, these signal probes were successfully utilized in the sensitive detection of PCV2 antibody by ELISA, fluorometry and square-wave voltammetry (SWV). To further demonstrate the performance of the immunosensor, Human IgG (HIgG) was used as a model analyte. Since more HRP and CdSe QDs were loaded, 5-, 200- and 400-fold enhancements in amplified ELISA, fluorometry and voltammetry responses for HIgG could be achieved compared to conventional ELISA. The respective detection limits of theses methods for HIgG were 3.9, 0.1 and 0.05 ng mL−1 with a RSD below 5% for amplified ELISA, fluorescence and SWV measurements. Additionally, a 100-fold improvement was obtained in the detection sensitivity for PCV2 antibody immunoassay. The versatile immunosensor exhibits good sensitivity, stability and reproducibility, suggesting its potential applications in clinical diagnostics.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 887, 5 August 2015, Pages 192–200
نویسندگان
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