Chemiluminescence lateral flow immunoassay based on Pt nanoparticle with peroxidase activity

• A lateral flow immunoassay (LFI) was developed with a chemiluminescent signal band.
• Pt nanoparticles were synthesized for the chemiluminescence reaction on the test strip.
• The peroxidase activity of Pt nanoparticles was optimized for the reaction conditions.
• The sensitivity of developed LFI was compared with the conventional colorimetric test.

A lateral flow immunoassay (LF-immunoassay) with an enhanced sensitivity and thermostability was developed by using Pt nanoparticles with a peroxidase activity. The Pt nanoparticles were synthesized by citrate reduction method, and the peroxidase activity of Pt nanoparticles was optimized by adjusting reaction conditions. The peroxidase activity was estimated by using Michaelis–Menten kinetics model with TMB as a chromogenic substrate. The kinetics parameters of KM and Vmax were calculated and compared with horseradish peroxidase (HRP). The thermal stability of the Pt nanoparticles was compared with horseradish peroxidase (HRP) according to the storage temperature and long-term storage period. The feasibility of lateral flow immunoassay with a chemiluminescent signal band was demonstrated by the detection of human chorionic gonadotropin (hCG) as a model analyte, and the sensitivity was determined to be improved by as much as 1000-fold compared to the conventional rapid test based on colored gold-colloids.

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