Ultrasonic microdialysis coupled with capillary electrophoresis electrochemiluminescence study the interaction between trimetazidine dihydrochloride and human serum albumin

• A homemade ultrasonic (US) microdialysis (MD) device was described.
• The equilibrium speed was improved about 6 times using USMD.
• The binding speed was improved 4 times using US technique.
• New method for the determination of binding sites number and binding constant was developed.

The paper describes a homemade ultrasonic microdialysis device coupled with capillary electrophoresis electrochemiluminescence (CE-ECL) for studying the interaction between human serum albumin (HSA) and trimetazidine dihydrochloride (TMZ). The time required for equilibrium by ultrasonic microdialysis was 45 min, which was far less than that by traditional dialysis (240 min). It took 80 min to achieve the required combination equilibrium by normal incubation and only 20 min by ultrasonic. Compared with traditional dialysis, the use of ultrasonic microdialysis simplified experimental procedures, shortened experimental time and saved consumption of sample. A simple, sensitive and selective determination of TMZ was developed using CE-ECL and the parameters that affected ECL intensity were optimized. Under the optimized conditions, the linear range of TMZ was from 0.075 to 80 μmol/L (r2 = 0.9974). The detection limit was 26 nmol/L with RSD of 2.8%. The number of binding sites and binding constant were 1.54 and 15.17 L/mol, respectively.

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