On-chip graphene oxide aptasensor for multiple protein detection

• On-chip aptasensor built on graphene oxide surface fixed on solid support.
• Multichannel configuration realizes simultaneous detection of 3–5 samples.
• Versatility of graphene oxide aptasensor formed on solid surface was studied.
• DNA and RNA aptamers immobilized on graphene oxide surface maintain their activity.
• Simultaneous multiple protein detection was demonstrated.

The versatility of an on-chip graphene oxide (GO) aptasensor was successfully confirmed by the detection of three different proteins, namely, thrombin (TB), prostate specific antigen (PSA), and hemagglutinin (HA), simply by changing the aptamers but with the sensor composition remaining the same. The results indicate that both DNA and RNA aptamers immobilized on the GO surface are sufficiently active to realize an on-chip aptasensor. Molecular selectivity and concentration dependence were investigated in relation to TB and PSA detection by using a dual, triple, and quintuple microchannel configuration. The multiple target detection of TB and PSA on a single chip was also demonstrated by using a 2 × 3 linear-array GO aptasensor. This work enables us to apply this sensor to the development of a multicomponent analysis system for a wide variety of targets by choosing appropriate aptamers.

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