کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1163998 1491013 2014 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Amorphous carbon nanoparticle used as novel resonance energy transfer acceptor for chemiluminescent immunoassay of transferrin
ترجمه فارسی عنوان
نانوذرات کربن آمورفیک به عنوان گیرنده انتقال انرژی جدید رزونانس برای آزمایش ایمونوفلورسانس انتقال دهنده مورد استفاده قرار می گیرد
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• Amorphous carbon nanoparticles (ACNPs) were prepared with a very simple protocol.
• The ACNP was employed as a novel energy acceptor for CRET immunosensing platform.
• The ACNPs showed higher energy transfer efficiency than graphene oxide.
• The proposed platform showed low cost, simple manipulation and high specificity.

Amorphous carbon nanoparticles (ACNPs) showing highly efficient quenching of chemiluminescence (CL) were prepared from candle soot with a very simple protocol. The prepared ACNP was employed as the novel energy acceptor for a chemiluminescence resonance energy transfer (CRET)-based immunoassay. In this work, ACNP was linked with transferrin (TRF), and horseradish peroxidase (HRP) was conjugated to TRF antibody (HRP–anti-TRF). The immunoreaction rendered the distance between the ACNP acceptor and the HRP-catalyzed CL emitter to be short enough for CRET occurring. In the presence of TRF, this antigen competed with ACNP–TRF for HRP–anti-TRF, thus led to the decreased occurrence of CRET. A linear range of 20–400 ng mL−1 and a limit of detection of 20 ng mL−1 were obtained in this immunoassay. The proposed method was successfully applied for detection of TRF levels in human sera, and the results were in good agreement with ELISA method. Moreover, the ACNPs show higher energy transfer efficiency than other conventional nano-scaled energy acceptors such as graphene oxide in CRET assay. It is anticipated that this approach can be developed for determination of other analytes with low cost, simple manipulation and high specificity.

Amorphous carbon nanoparticle was synthesized and used as a novel energy acceptor to develop a CRET-based immunoassay platform for biomolecule (TRF as the model) detection.Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 819, 28 March 2014, Pages 102–107
نویسندگان
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