کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1164092 1490974 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Rapid concentration of deoxyribonucleic acid via Joule heating induced temperature gradient focusing in poly-dimethylsiloxane microfluidic channel
ترجمه فارسی عنوان
غلظت سریع اسید دزوکسی ریبونوکلئیک از طریق گریدول حرارتی ناشی از حرارت جول با تمرکز در کانال مایع فلوئیدی پلی دی سیلکسان
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• Rapid microfluidic concentration enhancement of DNA with 480 folds in 40 s.
• A new numerical model for Joule heating induced TGF by combined AC and DC field.
• First demonstration of DNA concentration enhancement using Joule heating induced TGF.

This paper reports rapid microfluidic electrokinetic concentration of deoxyribonucleic acid (DNA) with the Joule heating induced temperature gradient focusing (TGF) by using our proposed combined AC and DC electric field technique. A peak of 480-fold concentration enhancement of DNA sample is achieved within 40 s in a simple poly-dimethylsiloxane (PDMS) microfluidic channel of a sudden expansion in cross-section. Compared to a sole DC field, the introduction of an AC field can reduce DC field induced back-pressure and produce sufficient Joule heating effects, resulting in higher concentration enhancement. Within such microfluidic channel structure, negative charged DNA analytes can be concentrated at a location where the DNA electrophoretic motion is balanced with the bulk flow driven by DC electroosmosis under an appropriate temperature gradient field. A numerical model accounting for a combined AC and DC field and back-pressure driven flow effects is developed to describe the complex Joule heating induced TGF processes. The experimental observation of DNA concentration phenomena can be explained by the numerical model.

Rapid microfluidic concentration enhancement of deoxyribonucleic acid (DNA) with Joule heating induced temperature gradient focusing under a combined AC and DC electric field.Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 858, 9 February 2015, Pages 91–97
نویسندگان
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