کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1164301 | 1491018 | 2014 | 8 صفحه PDF | دانلود رایگان |
• Single frequency impedance technique was firstly used for characterization of interaction between HER-3 and anti-HER-3.
• Anti-HER-3 antibody was firstly utilized in an immunosensor as a bioreceptor.
• The biosensor exhibits high analytical performance with a linear range 0.2–1.4 pg mL−1.
• Kramers–Kronig transform was successfully performed on the experimental impedance data.
We have developed a new immunological biosensor for ultrasensitive quantification of human epidermal growth factor receptor-3(HER-3). In order to construct the biosensor, the gold electrode surface was layered with, hexanedithiol, gold nanoparticles, and cysteamine, respectively. Anti-HER-3 antibody was covalently attached to cysteamine by glutaraldehyde and used as a bioreceptor in a biosensor system for the first time by this study. Surface characterization was obtained by means of electrochemical impedance spectroscopy and voltammetry. The proposed biosensor showed a good analytical performance for the detection of HER-3 ranging from 0.2 to 1.4 pg mL−1. Kramers–Kronig transform was performed on the experimental impedance data. Moreover, in an immunosensor system, the single frequency impedance technique was firstly used for characterization of interaction between HER-3 and anti-HER-3. Finally the presented biosensor was applied to artificial serum samples spiked with HER-3.
Figure optionsDownload as PowerPoint slide
Journal: Analytica Chimica Acta - Volume 814, 3 March 2014, Pages 31–38