Fabrication of glucose biosensor for whole blood based on Au/hyperbranched polyester nanoparticles multilayers by antibiofouling and self-assembly technique

Acknowledging the benefits of hyperbranched polymers and their nanoparticles, herein we report the design and synthesis of sulfonic acid group functionalized hydroxyl-terminated hyperbranched polyester (H30-SO3H) nanoparticles and their biomedical application. The H30-SO3H nanoparticles were characterized by transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectroscopy and proton nuclear magnetic resonance spectroscopy (1H NMR). The good hemocompatibility of H30-SO3H nanoparticles was also investigated by coagulation tests, complement activation and platelet activation. The novel glucose biosensor was fabricated by immobilizing the positively charged Au nanoparticles, H30-SO3H nanoparticles and glucose oxidase (GOx) onto the surface of glassy carbon electrode (GCE). It can be applied in whole blood directly, which was based on the good hemocompatibility and antibiofouling property of H30-SO3H nanoparticles. The biosensor had good electrocatalytic activity toward glucose with a wide linear range (0.2–20 mM), a low detection limit 1.2 × 10−5 M in whole blood and good anti-interference property. The development of materials science will offer a novel platform for application to substance detection in whole blood.

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• A novel method for detection of glucose in whole blood has been developed.
• The method based on antibiofouling and self-assembly technology was investigated.
• The antibiofouling technique utilized for sensor is significant for diagnostics.