کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1165019 1491061 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Simultaneous detection of lactate and glucose by integrated printed circuit board based array sensing chip
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Simultaneous detection of lactate and glucose by integrated printed circuit board based array sensing chip
چکیده انگلیسی

An integrated printed circuit board (PCB) based array sensing chip was developed to simultaneously detect lactate and glucose in mouse serum. The novelty of the chip relies on a concept demonstration of inexpensive high-throughput electronic biochip, a chip design for high signal to noise ratio and high sensitivity by construction of positively charged chitosan/redox polymer Polyvinylimidazole-Os (PVI-Os)/carbon nanotube (CNT) composite sensing platform, in which the positively charged chitosan/PVI-Os is mediator and electrostatically immobilizes the negatively charged enzyme, while CNTs function as an essential cross-linker to network PVI-Os and chitosan due to its negative charged nature. Additional electrodes on the chip with the same sensing layer but without enzymes were prepared to correct the interferences for high specificity. Low detection limits of 0.6 μM and 5 μM were achieved for lactate and glucose, respectively. This work could be extended to inexpensive array sensing chips with high sensitivity, good specificity and high reproducibility for various sensor applications.

Figure optionsDownload as PowerPoint slideHighlights
► An integrated printed circuit board (PCB) based array sensing chip was developed.
► Simultaneous detection of lactate and glucose in serum has been demonstrated.
► The array electronic biochip has high signal to noise ratio and high sensitivity.
► Additional electrodes were designed on the chip to correct interferences.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 771, 10 April 2013, Pages 102–107
نویسندگان
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