کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1165050 1491015 2014 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Extremely sensitive sandwich assay of kanamycin using surface-enhanced Raman scattering of 2-mercaptobenzothiazole labeled gold@silver nanoparticles
ترجمه فارسی عنوان
تست ساندویچ بسیار حساس کانامین سین با استفاده از پراکندگی رامان با افزایش سطح 2 نانو ذرات طلا و نقره با دوز مروارپتوبتنزو تیازول
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• Kanamycin immobilized hybrid magnetic nanoparticles were fabricated.
• They were sandwiched with the SERS substrate.
• The limit of detection was determined to be 2 pg mL−1.

Herein, we report the development of extremely sensitive sandwich assay of kanamycin using a combination of anti-kanamycin functionalized hybrid magnetic (Fe3O4) nanoparticles (MNPs) and 2-mercaptobenzothiazole labeled Au-core@Ag-shell nanoparticles as the recognition and surface-enhanced Raman scattering (SERS) substrate, respectively. The hybrid MNPs were first prepared via surface-mediated RAFT polymerization of N-acryloyl-l-glutamic acid in the presence of 2-(butylsulfanylcarbonylthiolsulfanyl) propionic acid-modified MNPs as a RAFT agent and then biofunctionalized with anti-kanamycin, which are both specific for kanamycin and can be collected via a simple magnet. After separating kanamycin from the sample matrix, they were sandwiched with the SERS substrate. According to our experimental results, the limit of detection (LOD) was determined to be 2 pg mL−1, this value being about 3–7 times more than sensitive than the LOD of previously reported results, which can be explained by the higher SERS activity of silver coated gold nanoparticles. The analysis time took less than 10 min, including washing and optical detection steps. Furthermore, the sandwich assay was evaluated for investigating the kanamycin specificity on neomycin, gentamycin and streptomycin and detecting kanamycin in artificially contaminated milk.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 817, 19 March 2014, Pages 33–41
نویسندگان
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