کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1165075 1491060 2013 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Aptamer based strategy for cytosensing and evaluation of HER-3 on the surface of MCF-7 cells by using the signal amplification of nucleic acid-functionalized nanocrystals
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Aptamer based strategy for cytosensing and evaluation of HER-3 on the surface of MCF-7 cells by using the signal amplification of nucleic acid-functionalized nanocrystals
چکیده انگلیسی

The electrochemical detection of cell lines of MCF-7 (human breast cancer) has been reported, using magnetic beads for the separation tool and high-affinity DNA aptamers for signal recognition. The high specificity was obtained by using the magnetic beads and aptamers, and the good sensitivity was realized with the signal amplification of DNA capped CdS or PbS nanocrystals. The ASV (anodic stripping voltammetry) technology was employed for the detection of cadmic cation and lead ions, for electrochemical assay of the amount of the target cells and biomarkers on the membrane of target cells, respectively. This electrochemical method could respond to as low as 100 cells mL−1 of cancer cells with a linear calibration range from 1.0 × 102 to 1.0 × 106 cells mL−1, showing very high sensitivity. Moreover, the amounts of HER-3 which were overexpressed on MCF-7 cells were calculated correspond to be 3.56 × 104 anti-HER-3 antibody molecules. In addition, the assay was able to differentiate between different types of target and control cells based on the aptamers and magnetic beads used in the assay, indicating the wide applicability of the assay for early and accurate diagnose of cancers.

An electrochemical strategy based on aptamers and nanoprobes was developed for the rapid collection and detection of MCF-7 cells and HER-3 on the surface of MCF-7 cells.Figure optionsDownload as PowerPoint slideHighlights
► Nanoparticles were used for the amplification of electrochemical detection.
► Magnetic beads (MBs) were used for the separation tool.
► High-affinity DNA aptamers were used for signal recognition.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 772, 15 April 2013, Pages 26–32
نویسندگان
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