کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1165774 | 1491112 | 2012 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: A dendrimer-based immunosensor for improved capture and detection of tumor necrosis factor-α cytokine A dendrimer-based immunosensor for improved capture and detection of tumor necrosis factor-α cytokine](/preview/png/1165774.png)
A dendrimer-based sandwich type enzyme-linked immunosorbent assay (ELISA) was developed for the improved detection of recombinant human tumor necrosis factor-alpha (TNF-α) for early diagnosis of perinatal diseases. Hydroxyl-terminated generation four poly(amidoamine) dendrimer (G4-OH) was used for the development of a solid phase bio-sensing platform. The surface of the ELISA plate was modified with polyethylene-glycol (PEG) and thiol-functionalized G4-OH was immobilized on the PEG-functionalized plate. A capture antibody was oxidized and covalently immobilized onto the dendrimer-modified ELISA plate, which provides favorable orientation for the antigen binding sites toward the analyte. The dendrimer-modified plate showed enhanced sensitivity, and the detection limit for TNF-α was found to be 0.48 pg mL−1, which is significantly better than the commercially available ELISA kit. The selectivity of the dendrimer-modified ELISA plate was further evaluated with a mixture of cytokines, which showed results for similar to that of TNF-α alone. The modified plate provides a greater opportunity for the detection of a wide range of cytokines and biomarkers.
Figure optionsDownload as PowerPoint slideHighlights
► Sensitive, early detection of cytokines can help in identifying perinatal diseases.
► Appropriately grafted dendrimer on the biosensor leads to better antibody orientation.
► Significantly improved detection limit and sensitivity has been shown for TNF-α.
► Platform approach is adaptable to both capture and detection, and for a wide variety of cytokines.
Journal: Analytica Chimica Acta - Volume 720, 30 March 2012, Pages 118–125