Enzyme activity measurement via spectral evolution profiling and PARAFAC

• Enzyme activity determination using FTIR and PARAFAC.
• Directly and universally applicable without the need of any external standards.
• The method provides alternative solutions to the cumbersome reducing sugar analysis.
• Enzymatic hydrolysis of plant polymers and sugar release can be determined.

The recent advances in multi-way analysis provide new solutions to traditional enzyme activity assessment. In the present study enzyme activity has been determined by monitoring spectral changes of substrates and products in real time. The method relies on measurement of distinct spectral fingerprints of the reaction mixture at specific time points during the course of the whole enzyme catalyzed reaction and employs multi-way analysis to detect the spectral changes. The methodology is demonstrated by spectral evolution profiling of Fourier Transform Infrared (FTIR) spectral fingerprints using parallel factor analysis (PARAFAC) for pectin lyase, glucose oxidase, and a cellulase preparation.

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