کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1165926 1491093 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of an automatic multi-channel ink-jet ejection chemiluminescence system and its application to the determination of horseradish peroxidase
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Development of an automatic multi-channel ink-jet ejection chemiluminescence system and its application to the determination of horseradish peroxidase
چکیده انگلیسی

In this work, an automatic multi-channel ink-jet for chemiluminescence (CL) analysis was developed. The four-channel ink-jet device was controlled by a home-made circuit. Differing from the classic flow injection CL, the whole procedure for CL analysis was automatically completed on a hydrophobic glass side. CL reaction of luminal and hydrogen peroxide for the determination of horseradish peroxidase (HRP) was selected as an application to automatic CL analysis platform. All solutions delivered by different channels were precisely ejected to the same position of the glass slide for the CL analysis. The consumption of reaction solution was reduced to nanoliter level. The whole CL analysis could be completed in less than 4 min, which was benefited from the prompt solution mixing in small size of droplet. The CL intensity increased linearly with HRP concentration in the range from 0.01 to 0.5 μg mL−1. The limit of detection (LOD) (S/N = 3) was 0.005 μg mL−1. Finally, the automatic CL system could also be used for the detection of HRP in HRP–protein conjugates, which showed its practical application in immunoassay.

An automatic multi-channel ink-jet ejection chemiluminescence system.Figure optionsDownload as PowerPoint slideHighlights
► Automatic multi-channel ink-jet ejection chemiluminescence.
► Determination of horseradish peroxidase.
► Horseradish peroxidase–protein conjugates.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 739, 20 August 2012, Pages 77–82
نویسندگان
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