کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1166434 | 1491102 | 2012 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Thermodynamic evaluation of the stability of the bone-seeking radiopharmaceutical [177Lu]Lu(III)–DOTP under simulated blood plasma conditions Thermodynamic evaluation of the stability of the bone-seeking radiopharmaceutical [177Lu]Lu(III)–DOTP under simulated blood plasma conditions](/preview/png/1166434.png)
The stability and in vivo robustness of [177Lu]Lu–DOTP as a potential bone-targeting radiopharmaceutical was determined with the aid of thermodynamic blood plasma modeling simulations. Glass electrode potentiometry was employed to measure the stability constants of the complexes of Lu3+ with DOTP. Similarly, the complexes of DOTP with a selection of the important physiological metal ions: Ca2+, Mg2+, and Cu2+ were determined, representing the typical interactions that the ligand would encounter upon administration. This made possible the construction of a blood plasma model of DOTP, aiding in establishing the potential susceptibility of the radiopharmaceutical. The ligand binds predominantly to calcium in vivo, accounting for 59.6% of that initially introduced as a component of the Lu–DOTP complex. Furthermore, due to a preference of the DOTP to bind to Cu2+ it causes mobilization of the ions in blood plasma, and would therefore indicate a deficiency if the ligand is administered at a concentration of 8.5 × 10−5 mol dm−3. The lutetium-ions are preferentially bound to DOTP, with as much as 98.1% of the Lu3+ occupying the ligand under physiological conditions.
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► Thermodynamic blood plasma model supports in vivo biodistribution of [177Lu]Lu–DOTP.
► Lu3+ remains predominantly complexed (98.1%) with the DOTP under simulated physiological conditions.
► Thermodynamic and kinetic stability [177Lu]Lu–DOTP in blood plasma facilitates selectivity of the proposed radiopharmaceutical for bone.
Journal: Analytica Chimica Acta - Volume 730, 12 June 2012, Pages 66–70