کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1166614 960505 2011 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Determination of thebaine in water samples, biological fluids, poppy capsule, and narcotic drugs, using electromembrane extraction followed by high-performance liquid chromatography analysis
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Determination of thebaine in water samples, biological fluids, poppy capsule, and narcotic drugs, using electromembrane extraction followed by high-performance liquid chromatography analysis
چکیده انگلیسی

Opium determination is of great importance from toxicological and pharmaceutical standpoints. In present work, electromembrane extraction (EME) coupled with high-performance liquid chromatography (HPLC) and ultraviolet (UV) detection was developed for determination of thebaine as a natural alkaloid, in different matrices containing water, urine, poppy capsule, street heroine, and codeine tablet. Thebaine migrated from 3 mL of sample solutions, through a thin layer of 2-nitrophenyl octyl ether (NPOE) immobilized in the pores of a porous hollow fiber, and into a 15 μL acidic aqueous acceptor solution present inside the lumen of the fiber. The variables of interest, such as chemical composition of the organic liquid membrane, stirring speed, extraction time and voltage, pH of donor and acceptor phases and salt effect in the EME process were optimized. Under optimal conditions, thebaine was effectively extracted from different matrices with recoveries in the range of 45–55%, which corresponded to preconcentration factors in the range of 90–110. Good linearity was achieved for calibration curves with a coefficient of estimation higher than 0.997. Detection limits and intra-day precision (n = 3) were less than 15 μg L−1 and 8.9%, respectively.

Figure optionsDownload as PowerPoint slideHighlights
► The opium determination is of a great deal of importance from toxicological and pharmaceutical standpoints.
► Thebaine, an opium alkaloid, has been suggested as a good urinary marker of poppy capsule use.
► Electromembrane microextraction method was coupled with high performance liquid chromatography and ultraviolet detection.
► The method was developed for determination of thebaine in water, urine, poppy capsule, street heroine, and codeine tablet.
► Detection limits and intra-day precision (n = 3) less than 15 μg L−1 and 8.9%, were obtained respectively.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 701, Issue 2, 9 September 2011, Pages 181–188
نویسندگان
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