A highly sensitive coupling technique for the determination of trace quercetin based on solid substrate room temperature phosphorimetry and poly (vinyl alcohol) complex imprinting

Al3+ could react with quercetin (Q) to form [AlQ]3+ complex which could be used as a template for the preparation of poly (vinyl alcohol)–[AlQ]3+ complex imprinting (PVA-C-I). The [AlQ]3+ not only had good matching ability and selectivity with the cavity of PVA-C-I, but also could react with the fluorescein isothiocyanate anion (FITC−) on the outside of cavity by electrostatic interaction to form ion-association complex [AlQ]3+·[(FITC)−]3. The ion-association complex could emit strong and stable room temperature phosphorescence (RTP) on polyamide membrane (PAM) and the ΔIp of the system had linear relationship with the content of Q, showing the highly selective identification of PVA-C-I to Q. Thus, a new coupling technique for the determination of trace Q based on solid substrate room temperature phosphorimetry and poly (vinyl alcohol) complex imprinting (PVA-C-I-SSRTP) was established. The linear range and limit of detection (LOD) of this method were 0.010–2.0 (×10−12 g mL−1) and 2.0 × 10−14 g mL−1, respectively, showing wide linear range and high sensitivity of PVA-C-I-SSRTP. This method was used to determine the content of Q in waste water, and the results are consistent with those by spectrofluorimetry. Meanwhile, the mechanism for the determination of Q using PVA-C-I-SSRTP was also discussed.

In this paper, [AlQ]3+ had good selectivity with the hole of PVA-C-I and could react with the fluorescein isothiocyanate anion on the outside of hole by electrostatic interaction to form ion-association complex [AlQ]3+·[FITC]−3. Thus, a new coupling technique for the determination of trace Q based on solid substrate room temperature phosphorimetry and poly (vinyl alcohol) complex imprinting was established.Figure optionsDownload as PowerPoint slideHighlights
► The phosphorimetry-poly (vinyl alcohol)-complex imprinting coupling technique (PVA-C-I-RTP) was exploited.
► The phosphorimetry with the technology of PVA-C-I for the determination of trace quercetin was established.
► This technology could identify quercetin, which could be used to separate, enrich and determine quercetin.
► The mechanism for the determination of quercetin using PVA-C-I-RTP was also discussed.
► This work promoted the research progress and the interpenetration between complex imprinting technique and SSRTP.