کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1167373 960540 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Amperometric sensor for cyanide utilizing cyanidase and formate dehydrogenase
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Amperometric sensor for cyanide utilizing cyanidase and formate dehydrogenase
چکیده انگلیسی

An amperometric sensor for the detection of cyanide integrated in a flow injection analysis (FIA) system is presented. The specificity of the sensor for cyanide is achieved by a reaction cascade involving two enzymes, cyanidase (EC 3.5.5.1) and formate dehydrogenase (FDH, EC 1.2.1.2). The limit of detection (LOD) and the limit of quantification (LOQ) were determined to be in the low micromolar range. The sensor showed very good long term stability and the linear range of detection extends from 0.7 up to 800 μM. The sensor’s ability to measure cyanide in complex matrices, such as plant extracts, is demonstrated. Although the sensor is susceptible to formate and to a lesser extent to thiocyanate, sulfide does not interfere significantly.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 673, Issue 1, 12 July 2010, Pages 54–59
نویسندگان
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