کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1167947 1491140 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development and application of a quantitative lateral flow immunoassay for fumonisins in maize
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Development and application of a quantitative lateral flow immunoassay for fumonisins in maize
چکیده انگلیسی

A quantitative lateral flow immunoassay for measuring fumonisins in maize was developed. Strip preparation and assay parameters were optimized to obtain a dipstick usable outside the laboratory with different samples, and which shows performance comparable with that of other screening methods, as confirmed by the intra- and the inter-day precision of data (RSD 5–16%). Quantification was obtained by an external calibration curve, which can be stored and used for measurements made with strips of the same batch in different days and at varying temperatures (22–37 °C). Limit of detection (120 μg L−1) and dynamic range (200–5000 μg L−1) allow the direct assessment of fumonisin contamination at all levels of regulatory relevance. Twenty-seven maize samples were analyzed after a simple sample preparation which avoids the use of organic solvent. Linear correlation was observed (y = 1.071x − 0.2, r2 = 0.990) when data was compared with that obtained through a reference LC–MS/MS method, across a wide range of fumonisin contamination.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 682, Issues 1–2, 3 December 2010, Pages 104–109
نویسندگان
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