کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1168501 | 960592 | 2009 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Metabolomic profiling of human urine in hepatocellular carcinoma patients using gas chromatography/mass spectrometry Metabolomic profiling of human urine in hepatocellular carcinoma patients using gas chromatography/mass spectrometry](/preview/png/1168501.png)
With the technique of metabolomics, gas chromatography/mass spectrometry (GC/MS), urine or serum metabolites can be assayed to explore disease biomarkers. In this work, we present a metabolomic method to investigate the urinary metabolic difference between hepatocellular carcinoma (HCC, n = 20) male patients and normal male subjects (n = 20). The urinary endogenous metabolome was assayed using chemical derivatization followed by GC/MS. After GC/MS analysis, 103 metabolites were detected, of which 66 were annotated as known compounds. By a two sample t-test statistics with p < 0.05, 18 metabolites were shown to be significantly different between the HCC and control groups. A diagnostic model was constructed with a combination of 18 marker metabolites or together with alphafetoprotein, using principal component analysis and receiver–operator characteristic curves. The multivariate statistics of the diagnostic model yielded a separation between the two groups with an area under the curve value of 0.9275. This non-invasive technique of identifying HCC biomarkers from urine may have clinical utility.
Journal: Analytica Chimica Acta - Volume 648, Issue 1, 19 August 2009, Pages 98–104