کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1169243 | 960626 | 2008 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Assays for serum cholinesterase activity by capillary electrophoresis and an amperometric flow injection choline biosensor
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Assays for serum cholinesterase activity by capillary electrophoresis and an amperometric flow injection choline biosensor Assays for serum cholinesterase activity by capillary electrophoresis and an amperometric flow injection choline biosensor](/preview/png/1169243.png)
چکیده انگلیسی
The FIA-choline sensor was constructed with the working electrode of the flow cell covered with a natural chitinous membrane purified from Taiwanese soldier crab, Mictyris brevidactylus. The biomembrane served as the supporting material for enzyme immobilization (choline oxidase, EC 1.1.3.17), and also prevented protein adsorption on the electrode surface. The calibration curve was linear between 0.05 and 5.0 mM (r = 0.999). The relative standard deviations for 1.0 mM choline (n = 7) were less than 3%, and the activity of the bioactive membrane lasted for about 2 months. The analytical results of both methods correlated well (r = 0.940).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 623, Issue 2, 15 August 2008, Pages 157-162
Journal: Analytica Chimica Acta - Volume 623, Issue 2, 15 August 2008, Pages 157-162
نویسندگان
Bo-Chuan Hsieh, Hsien-Yi Hsiao, Tzong-Jih Cheng, Richie L.C. Chen,