کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1170427 1491173 2007 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Application of chemometric tools for automatic classification and profile extraction of DNA samples in forensic tasks
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Application of chemometric tools for automatic classification and profile extraction of DNA samples in forensic tasks
چکیده انگلیسی
In this paper a method for the automatic DNA spots classification and extraction of profiles associated in DNA polyacrilamide gel electrophoresis is presented and it integrates the use of image processing techniques and chemometrics tools. A software which implements this method was developed; for feature extraction a combination of a PCA analysis and a C4.5 decision tree were used. To obtain good results in the profile extraction only DNA spots are useful; therefore, it was necessary to solve a two-class classification problem among DNA spots and no-DNA spots. In order to perform the classification process with high velocity, effectiveness and robustness, comparative classification studies among support vector machine (SVM), K-NN and PLS-DA classifiers were made. The best results obtained with the SVM classifier demonstrated the advantages attributed to it in the literature as a two-class classifier. A Sequential Cluster Leader Algorithm and another one developed for the restoration of pattern missing spots were needed to conclude the profiles extraction step. The experimental results show that this method has a very effective computational behavior and effectiveness, and provide a very useful tool to decrease the time and increase the quality of the specialist responses.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 595, Issues 1–2, 9 July 2007, Pages 43-50
نویسندگان
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