کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1170882 960694 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Optimization of separation and detection of 6-aminoquinolyl derivatives of amino acids by using reversed-phase liquid chromatography with on line UV, fluorescence and electrochemical detection
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Optimization of separation and detection of 6-aminoquinolyl derivatives of amino acids by using reversed-phase liquid chromatography with on line UV, fluorescence and electrochemical detection
چکیده انگلیسی

The combined use of UV-absorbance, fluorescence and electrochemical detection was proposed for the analysis of a set of thirteen amino acids by reversed-phase liquid chromatography (RP-HPLC) using 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate as a precolumn derivatization reagent. The utility of using three detectors in series was demonstrated. The separation of all derivatized amino acids was optimized with the aid of a computer optimization program from only four simple linear gradient measurements. The effectiveness of a reliable retention prediction of solutes under any gradient profile using other gradient or isocratic data was also examined.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 593, Issue 1, 12 June 2007, Pages 92–97
نویسندگان
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