کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1170944 960697 2006 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Determination of risedronate in rat plasma samples by ion-pair high-performance liquid chromatography with UV detector
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Determination of risedronate in rat plasma samples by ion-pair high-performance liquid chromatography with UV detector
چکیده انگلیسی

In the present work, an analytical method for determination of risedronate, a member of bisphosphonates, is described for the routine analysis in rat plasma. Sample pre-treatment involves protein precipitation, co-precipitation with calcium at alkaline pH, hydrolysis of possible derivatives of pyrophosphate and reprecipitation. A good separation was obtained by using a reversed-phase column (Hypersil ODS-2 C18, 4.6 mm × 250 mm, 5 μm). The mobile phase was an aqueous solution of buffer (contained 1.5 mM EDTA-2Na, 1 mM sodium etidronate, 11 mM sodium phosphate and 5 mM tetrabutylammonium bromide as ion-pair reagent) – methanol (88:12, v/v) adjusted to pH 6.75 using 1 M NaOH. The flow rate was 1 ml min−1. UV detection (λ = 262 nm) was used to quantitate risedronate in the concentration range of 10–500 ng ml−1. The limit of detection and quantitation for risedronate were 7 and 10 ng ml−1, respectively. The method was applied successfully to plasma samples from Wistar rats undergoing oral administration of risedronate mini-pills. Precision, extraction recoveries, as well as accuracy results, were satisfactory and no interference was found at the retention time of risedronate. Hence, the method is suitable for monitoring risedronate in rat plasma.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 562, Issue 2, 15 March 2006, Pages 171–175
نویسندگان
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