کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1171877 | 960734 | 2006 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: A simple and rapid method for identification and determination of cordycepin in Cordyceps militaris by capillary electrophoresis A simple and rapid method for identification and determination of cordycepin in Cordyceps militaris by capillary electrophoresis](/preview/png/1171877.png)
Cordycepin is the main active metabolite of Cordyceps militaris extracts; according to recent studies it has interesting therapeutic activities. A new capillary electrophoresis (CE) procedure with UV detection at 254 nm for determination of cordycepin was developed and optimized. Optimal conditions found were 20 mM sodium borate buffer with 28.6% methanol, pH 9.5, separation voltage 20 kV, hydrodynamic injection time 10 s and temperature 25 °C. Linearity was found over the 20–100 μg/mL concentration ranges of cordycepin. The developed method has been applied for determination of cordycepin in various pharmaceutical products. A comparison was made between CE and a high performance liquid chromatography (HPLC) method. Both of these methods gave comparable results. The shorter analysis time and low running cost are the main advantages of CE method.
Journal: Analytica Chimica Acta - Volume 566, Issue 2, 4 May 2006, Pages 253–258