کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1171932 | 960737 | 2006 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Highly sensitive fluorescence detection with Hg-lamp and photon counter in microchip capillary electrophoresis
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Highly sensitive fluorescence detection with Hg-lamp and photon counter in microchip capillary electrophoresis Highly sensitive fluorescence detection with Hg-lamp and photon counter in microchip capillary electrophoresis](/preview/png/1171932.png)
چکیده انگلیسی
A microchip capillary electrophoresis system with highly sensitive fluorescence detection is reported. The system was successfully constructed using an inverted fluorescence microscope, a highly sensitive photon counter, a photomultiplier tube (PMT) and a capillary electrophoresis microchip. This system can be applied to the fluorescence detection with various wavelengths (300-600Â nm). Different fluorescence reagents require different excitation wavelengths. The wavelengths of UV light (300-385Â nm), blue light (450-480Â nm) and green light (530-550Â nm) are employed to excite Titan yellow, fluorescence-5-isothiocyanate (FITC) and Rhodamine 6G, respectively. The detection limit (S/NÂ =Â 3) of FITC is 7Â ÃÂ 10â10Â M, which is 2-3 orders of magnitude lower than that obtained with the lamp-based fluorescence and PMT detection system and approaches the data gained by the laser-induced fluorescence detection. The linear relationship is excellent within the range of concentration 1.3Â ÃÂ 10â9 to 6.5Â ÃÂ 10â8Â M FITC. It offers a new method to widen the application of the lamp-based fluorescence detection.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 555, Issue 2, 12 January 2006, Pages 246-249
Journal: Analytica Chimica Acta - Volume 555, Issue 2, 12 January 2006, Pages 246-249
نویسندگان
Qin Yan, Rong Sheng Chen, Jie-Ke Cheng,