کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1171972 960738 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Monolithic molecularly imprinted polymer for sulfamethoxazole and molecular recognition properties in aqueous mobile phase
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Monolithic molecularly imprinted polymer for sulfamethoxazole and molecular recognition properties in aqueous mobile phase
چکیده انگلیسی

A monolithic molecularly imprinted polymer (monolithic MIP) for sulfamethoxazole (SMO) was prepared by in situ polymerization method as the HPLC stationary phase. By optimizing the polymerization conditions, the monolithic MIP showed highly specific recognition for the template SMO over its three structurally related analogs. As shown by SEM and the pore size distribution profile, the resultant MIP monolith showed a main pore diameter of 594 nm and a large specific surface area of 124 m2 g−1, this allowed the mobile phase to flow through the column with low backpressure. Furthermore, the recognition abilities of the monolithic MIP in aqueous and organic media were studied. The results exhibited that the monolithic MIP possessed excellent recognition ability in aqueous media. Hydrophobic interactions, in addition to shape recognition, were the dominant effect for recognition in the mobile phase with high water content. Moreover, the binding sites and the dissociation constant were also determined by frontal chromatography as 122 μmol g−1 and 1.88 × 10−5 mol L−1, respectively, which demonstrated that the obtained SMO-MIP monolith had a high binding capacity and strong affinity ability to the template molecule. Furthermore, the resultant SMO-MIP monolith was used as HPLC column directly to determine the SMO contents in three kinds of pharmaceutical tablets with the optimized aqueous mobile phase.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 571, Issue 2, 7 July 2006, Pages 235–241
نویسندگان
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